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KMID : 0361620060410061021
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Journal of the Korean Orthopaedic Association
2006 Volume.41 No. 6 p.1021 ~ p.1027
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The Effect of Hyaluronic Acid on the Expression of MMP-13 in Healing Process of Bone Defect in Rats
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Kang Ki-Ser
Lee Han-Jun
Lee Jin-Woo
Lee Jae-Sung
Lee Mi-Kyung
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Abstract
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Purpose: Matrix metalloproteinase (MMP)-13 has a degradation effect on type II collagen and is expressed in osteoblastic cells and hypertrophic chondrocytes. However, the role of MMP-13 is not completely understood. This study investigated the effect of exogenous Hyaluronic acid (HA) on the expression of MMP-13 in the healing process of bone defects.
Materials and Methods: After making a bone defect in the metaphysis of the distal femur in rats, HA was instilled into the defect in the experimental group. In the control group, a bone defect was made but HA was not instilled. Two weeks after the experiment, the rats were sacrificed and the callus that filled the bone defects was obtained. A histological examination and real time reverse transcription polymeric chain reaction (RT-PCR) were performed to investigate the healing response and expression of MMP-13.
Results: Histologically, the proliferation of osteoblasts and the formation of osteoid were more active in the experimental group. Nevertheless, the level of MMP-13 expression was significantly lower in the experimental group than in the control group.
Conclusion: From the results of this study, it appears that exogenous HA inhibits the expression of MMP-13 at the early stage of bone healing.
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KEYWORD
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Bone defect, Healing, MMP-13, Hyaluronic acid
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